Human eye has distinct anatomy which makes it different from other body organs. It offers several barriers to effectively deliver the drug to the desired site. Ophthalmic gel forming solutions are one such novel dosage form which remain in solution form at room temperature and at physiological conditions they undergo sol to gel transition upon contact with lacrimal fluid. The objective of the current investigation was to formulate gel forming solution of ofloxacin using Gellan gum for controlled action in the management of bacterial infections. Gellan gum was dissolved in distilled water to create a polymeric solution, which was then used to develop the formulation intended in this study. A total of six different batches containing various concentrations of gellan gum were prepared. Drug and other excipients such as mannitol, and Benzdodecinium bromide were added employing constant stirring to obtain a slightly viscous solution and pH were adjusted accordingly. Numerous characteristics such as rheological behaviour, pH, in-vitro gelling capability, drug release was assessed for the developed formulations. Based on various evaluation parameters, F5 turned out to be the best formulation amongst three formulations developed with gelation time of 10 seconds and the gel remained intact for 8 hours. The rheological behaviour of the developed formulation F5 was found to be pseudoplastic with shear thinning nature. Additionally, it was able to release 94.06% of the drug in 8 hours during the in-vitro release trial, indicating prolonged release. During histopathology study, no signs of inflammation were noted. Obtained results were satisfactory and were in specified limits. Thus, it can be concluded that the prepared formulation F5 can be further explored for effective management of bacterial infections.